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Description
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Recent advances in molecular and cell biology enabling
the cloning, expression, and mutagenesis of signal transduction
proteins has prompted an explosion of knowledge in the field of
receptor regulation, facilitating the discovery of new classes of
regulatory proteins, and providing a basis and means for manipulating
receptor function through multiple intracellular targets.
This volume covers methods used to examine how the function(s) of
receptors are regulated. Understanding how to regulate the function and
expression of these receptors is critical in determining how to
modify receptors and to translocating receptors away from the cell
surface and its recycling.
Individual chapters focus on specific techniques used to characterize
receptors (epitope tagging, measurement and analysis of receptor
phosphorylation, analysis of the kinetics of receptor
desensitization, and assessment of receptor/G protein coupling); the
role of regulatory proteins (receptor kinases and phosphatases,
arrestins) in modulating receptor function; and the methods used to
measure receptor trafficking (ligand binding, immunofluoresence) and
expression (transcriptional and translational regulation).
* Covers a broad range of important concepts and methodologies which
are current in the study of G protein-coupled receptors (GPCRs)
* G-protein coupled receptors make up over 40% of the current
pharmacological targets
* Provides detailed protocols for executing various strategies and
offers informed judgments as to what approaches are and aren't useful
* Volume Editor, Jeffrey Benovic, is a dominant world leader in the
study of receptor regulation of GPCR kinases and is highly respected
in the field
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