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Description
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The detection of neurotransmitter receptor locations
and distribution densities within the central nervous system and
peripheral tissues is receiving intense attention within the
neuroscience research community. Neurotransmitter receptors, which
receive the chemical signals sent from one neuron to another, are
critical links in a highly complex information-processing chain.
Pinpointing receptor sites and systems is crucial for understanding
neurological function as well as dysfunction. It is also essential
for understanding how receptors process information when impacted
by such substances as heroin or nicotine, or when affected by
neurodegenerative disease.
Receptor Localization: Laboratory Methods and Procedures
is the first user-friendly guide to the latest techniques and
approaches being employed to examine the localization of
neurotransmitter receptors in the central nervous system and
peripheral tissues. It covers detection methods that are applicable
to a wide variety of receptor systems, ranging from genes and
ligands to in vito receptors in individuals; and to numerous
receptor subtypes, such as nicotine, muscarine, tackykinins,
dopamine, adenosine, and GABA. The standard laboratory
"recipes" or "tricks" employed in these detection
methods are fully discussed, as are the advantages and limitations
of each procedure. With contributions from leading experts and
extensively illustrated, this book:
- Discusses
receptor ligand binding methods using irreversible and
reversible compounds
- Presents
antireceptor antisera technology using synthetic peptides and
fusion proteins at both the cellular and subcellular
resolution levels
- Examines
molecular assessments of receptors
- Describes
in situ hybridization, reverse transcriptase-PCR, and
fluorescent in situ transcription
- Covers
new visualization paradigms
- Includes
physiological analysis of receptor function, cellular
detection in the brain slice, and cultured neurons
- Discusses
the use of PET and SPECT to assess in vivo receptor
distributions in animals and humans
Receptor Localization: Laboratory Methods and Procedures
is an invaluable guide for researchers in the related fields of
neurology, biochemistry, and pharmacology. Its lucid descriptions
of new detection methods, inclusion of experimental examples, and
emphasis on how these experimental approaches are applicable to
particular research areas will appeal to both the experienced
researcher and novice investigator.
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