|
|
Update May, 2002
Current Protocols in Food Analytical Chemistry is updated quarterly. Each supplement insert includes new as well as revised units to expand the scope of the publication and keep pace with new developments and advances in food analytical chemistry. This table of contents is revised as each supplement update is published to reflect new contents.
--------------------------------------------------------------------------------
Foreword
Preface
Contributors
A Water
B Proteins
C Enzymes
D Lipids
E Carbohydrates
F Pigments and Colorants
G Flavors
H Textural/Rheology
Appendices
Index
--------------------------------------------------------------------------------
A Water
A1 Gravimetric Measurements of Water
A1.1 Gravimetric Determination of Water by Drying and Weighing
A1.2 Karl Fischer Titration
A1.3 Application of Low-Resolution NMR for Simultaneous Moisture and Oil Determination
in Food (Oilseeds)
A2 Vapor Pressure Measurements of Water
A2.1 Factors to Consider When Estimating Water Vapor Pressure
A2.2 Dew-Point Method for the Determination of Water Activity
A2.3 Measurement of Water Activity using Isopiestic Methods
A2.4 Direct Manometric Determination of Vapor Pressure
A2.5 Measurement of Water Activity by Electronic Sensors (New, February 2002)
B Proteins
B1 Measurement of Protein Content
B1.1 The Colorimetric Detection and Quantitation of Total Protein
B1.2 Determination of Total Nitrogen
B1.3 Spectrophotometric Determination of Protein Concentration (New, May 2002)
B2 Biochemical Compositional Analyses of Proteins
B2.1 Analyses of Protein Quality
B2.2 Evaluation of the Progress of Protein Hydrolysis (New, May 2002)
B3 Characterization of
Proteins
B3.1 Electrophoresis Analysis
B3.2 Electroblotting from Polyacrylamide Gels
B3.3 Detection of Proteins on Blot Membranes
B3.4 Immunoblot Detection
B4 Purifcation of Proteins
B4.1 Overview of Protein Purification and Characterization (New, February 2002)
B4.2 Overview of Conventional Chromatography (New, February 2002)
C Enzymes
C1 Strategies For Enzyme Activity Measurements
C1.1 Strategies for Enzyme Activity Measurements
C2 Proteolytic Enzymes
C2.1 Activity Measurements of Proteases using Synthetic Substrates
C2.2 Peptidase Activity Assays Using Protein Substrates (New, February 2002)
C3 Lipolytic Enzymes
C3.1 Lipase Assays
C4 Oxidoreductases
C4.1 Polarographic and Spectrophotometric Assay of Diphenol Oxidases (Polyphenol
Oxidase)
C4.2 Analysis of Lipoxygenase Activity and Products
D Lipids
D1 Lipid Composition
D1.1 Extraction and Measurement of Total Lipids
D1.2 Analysis of Fatty Acids in Food Lipids
D1.3 Cholesterol
D1.4 Oil Quality Indices
D1.5 Analysis of Tocopherols and Tocotrienols (New, May 2002)
D2 Lipid Oxidation/Stability
D2.1 Measurement of Primary Lipid Oxidation Products
D2.2 Chromatographic Analysis of Secondary Lipid Oxidation Products
D2.3 Measurement of Oil Stability for Lipids
D2.4 Spectrophotometric Measurement of Secondary Lipid Oxidation Products
D3 Physical Properties
of Lipids
D3.1 Determination of Solid Fat Content by Nuclear Magnetic Resonance
D3.2 Lipid Crystal Characterization
D3.3 Emulsion Droplet Size Determination
D3.4 Emulsion Stability Determination (New, February 2002)
E Carbohydrates
E1 Mono- and Oligosaccharides
E1.1 Colorimetric Analyses
E2 Starch and Starch Derivatives
E2.1 Overview of Laboratory Isolation of Starch from Plant Materials
E2.2 Enzymatic Quantitation of Total Starch in Plant Products
E2.3 Determination of Total Amylose Content of Starch
E3 Cell Wall Polysaccharides
E3.1 Isolation of Plant Cell Walls and Fractionation of Cell Wall Polysaccharides
E3.2 Determination of Neutral Sugars by Gas Chromatography of their Alditol
Acetates
E3.3 Determination of the Uronic Acid Content of Plant Cell Walls Using a Colorimetric
Assay
E3.4 Determining the Degree of Methylation and Acetylation of Pectin
F Pigments and Colorants
F1 Anthocyanins
F1.1 Extraction, Isolation and Purification of Anthocyanins
F1.2 Characterization and Measurement of Anthocyanins by UV-Visible Spectroscopy
F1.3 Separation and Characterization of Anthocyanins by HPLC
F2 Carotenoids
F2.1 Extraction, Isolation and Purification of Carotenoids
F2.2 Detection and Measurement of Carotenoids by UV/VIS Spectrophotometry
F2.3 Chromatographic Techniques for Carotenoid Separation
F2.4 Mass Spectroscopy of Carotenoids
F3 Miscellaneous Colorants
F3.1 Betalains
F3.2 Spectrophotometric and Reflectance Measurements of Pigments of Cooked and
Cured Meats
F3.3 Measurement of Discoloration in Fresh Meat
F4 Chlorophylls
F4.1 Overview of Chlorophylls in Foods
F4.2 Extraction of Photosynthetic Tissues: Chlorophylls and Carotenoids
F4.3 Chlorophylls and Carotenoids: Measurement and Characterization by UV-VIS
Spectroscopy
F4.4 Chromatographic Separation of Chlorophylls
F4.5 Mass Spectrometry of Chlorophylls
F5 Strategies for Measuring
Colors and Pigments
F5.1 Overview of Color Analysis (New, February 2002)
G Flavors
G1 Smell Chemicals
G1.1 Direct Sampling
G1.2 Isolation and Concentration of Aroma Compounds
G1.3 Identification and Quantitation of Aroma Compounds
G1.4 Stereodifferentiation of Chiral Odorants Using High-Resolution Gas Chromatography
G2 Acid Tastants
G2.1 Titratable Activity of Acid Tastants
G2.2 Liquid Chromatography of Nonvolatile Acids
H Textural/Rheology
H1 Viscosity of Liquids, Solutions, and Fine Suspensions
H1.1 Overview of Viscosity and Its Characterization
H1.2 Measurement of Viscosity of Non-Newtonian Fluids
H1.3 Viscosity Determination of Pure Liquids, Solutions, and Serums Using Capillary
Viscometry
H1.4 Measuring Consistency of Juices and Pastes
H2 Compressive Measurement of Solids and Semi-Solids
H2.1 General Compressive Measurements
H2.2 Textural Measurements with Special Fixtures
H2.3 Texture Profile Analysis
Appendices
A.1 Abbreviations and Useful Data
A Abbreviations Used in This Manual
A.2 Laboratory Stock Solutions, Equipment, and Guidelines
A Common Buffers and Stock Solutions
B Laboratory Safety
C Standard Laboratory Equipment
A.3 Commonly Used Techniques
A Introduction to Mass Spectrometry for Food Chemistry
Supplier Appendix
Index