Table of Contents
Last Updated August, 1999
Foreword by
Roger O. McClellan
1 Toxicological Models
2 Assessment of Cell Toxicity
3 Genetic Toxicology: Mutagenesis and Adduct Formation
4 Techniques for Analysis of Chemical Biotransformation
5 Toxicokinetics
6 The Glutathione Pathway
7 Assessment of the Activity of Antioxidant Enzymes
8 Heme Synthesis Pathway
9 Heme Degradation Pathway
10 The Nitric Oxide/Guanylate Cyclase Pathway
11 Neurotoxicology
Appendices
Index
1 Toxicological Models
1.1 Nonhuman Primates as Animal Models for Toxicology Research
1.2 Statistical Approaches to the Design of Toxicology Studies
1.3 Transgenic Animals in Toxicology
2 Assessment of Cell Toxicity
2.1 Current Concepts in Cell Toxicity
2.2 Determination of Apoptosis and Necrosis
2.3 Detection of Covalent Binding
2.4 Measurement of Lipid Peroxidation
2.5 Measurements of Intracellular Free Calcium Concentration in Biological
Systems (New, August 99)
3 Genetic Toxicology: Mutagenesis and Adduct Formation
3.1 The Salmonella (Ames) Test for Mutagenicity
3.2 Measurement of a Malondialdehyde-DNA Adduct
3.3 Mutagenesis Assays in Mammalian Cells (New,
August 99)
3.4 Cell Transformation Assays (New, August
99)
4 Techniques for Analysis of Chemical Biotransformation
4.1 Measurement of Cytochrome P-450
4.2 Purification of Cytochrome P-450 Enzymes
4.3 Measurements of UDP-Glucuronosyltransferase (UGT) Activities
5 Toxicokinetics
5.1 Measurement of Bioavailability: Measurement of Absorption Through Skin
In Vitro
5.2 Measurement of Bioavailability: Measuring Absorption Through Skin
In Vivo in Rats and Humans
6 The Glutathione Pathway
6.1 Overview of Glutathione Function and Metabolism
6.2 Measurement of Glutathione and Glutathione Disulfide
6.3 Measurement of Glutathione Transport
6.4 Measurement of Glutathione Transferases
6.5 HPLC-Base Assays for Enzymes of Glutathione Biosynthesis
7 Assessment of the Activity of Antioxidant Enzymes
7.1 Analysis of Glutathione-Related Enzymes
7.2 Measurement of Glutathione Reductase Activity
7.3 Analysis of Superoxide Dismutase Activity
8 Heme Synthesis Pathway
8.1 The Heme Biosynthesis Pathways and Clinical Manifestations of Abnormal
Function
8.2 Measurement of ALA Synthase Activity
8.3 Measurement of Heme Concentration
8.4 Measurement of Uroporphyrinogen Decarboxylase Activity
8.5 Measurement of Protoporphyrinogen Oxidase Activity
8.6 Measurement of d-Aminolevulinate Dehydratase
Activity (New, August 99)
9 Heme Degradation Pathway
9.1 Overview of Heme Degradation Pathway
9.2 Detection of Heme Oxygenase Activity by Measurement of CO
9.3 Detection of Heme Oxygenase 1 and 2 Proteins and Bilirubin Formation
9.4 Detection of Biliverdin Reductase Activity
9.5 Histochemical Analysis of Heme Degradation Enzymes (New,
August 99)
10 The Nitric Oxide/Guanylate Cyclase Pathway
10.1 Overview of the Pathway and Functions of Nitric Oxide
10.2 Assay of Tissue Activity of Nitric Oxide Synthase
10.3 Detection of Nitrosated Proteins
10.4 Fluorometric Techniques for the Detection of Nitric Oxide and
Metabolites
10.5 Measurement of cGMP and Soluble Guanylyl Cyclase Activity
10.6 Histochemical Analysis of Nitric Oxide Synthase by NADPH Diaphorase
Staining (New, August 99)
11 Neurotoxicology
11.1 Overview of Neurotoxicology
11.2 Neurobehavioral Screening in Rodents
11.3 Assessment of Spatial Memory
11.4 Advanced Behavioral testing in Rodents: Assessment of Cognitive
Function in Animals
11.5 Testing for Organophosphate-Induced Delayed Polyneuropathy
Appendices
A.1 USEFUL INFORMATION
A. Safe Use of Radioisotopes
B. Transgenic and Gene-Targeted Mouse Lines for Toxicology Studies
(New, August 99)
A.2 LABORATORY STOCK SOLUTIONS AND EQUIPMENT
A. Common Stock Solutions and Buffers
B. Standard Laboratory Equipment
A.3 COMMONLY USED TECHNIQUES
A. Molecular Biology Techniques
Suppliers Appendix
