Table of Contents
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Chapter 1 Emerging Technologies
- Introduction
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(41K)
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Section A Safety
- Unit 1A.1 Biosafety: Guidelines for Working
with Pathogenic and Infectious Microorganisms
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(75K)
- Unit 1A.2 Biosafety Practices Associated
with Potential Agents of Biocrime and Biowarfare
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(217K)
- Unit 1A.3 Safe Use of Hazardous Chemicals
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(193K)
- Unit 1A.4 Safe Use of Radioisotopes
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(303K)
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Section B Biofilms
- Unit 1B.1 Growing and Analyzing Static
Biofilms
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(540K)
- Unit 1B.2 Growing and Analyzing Biofilms in
Flow Cells
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(150K)
- Unit 1B.3 Growing and Analyzing Biofilms in
Fermenters
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(175K)
- Unit 1B.5 Growing Oral Biofilms in a
Constant Depth Film Fermentor (CDFF)
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(831K)
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Section C Quorum Sensing
- Unit 1C.1 Methods for Analysis of Bacterial
Autoinducer-2 Production
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(88K)
- Unit 1C.2 LacZ-Based
Detection of Acyl-Homoserine Lactone Quorum-Sensing Signals
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(156K)
- Unit 1C.3 Detection and Analysis of
Quorum-Quenching Enzymes Against Acyl Homoserine Lactone Quorum-Sensing
Signals
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(297K)
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Section D PCR Techniques
- Unit 1D.3 Example of Real-Time Quantitative
Reverse Transcription–PCR (Q-RT-PCR) Analysis of Bacterial Gene
Expression during Mammalian Infection: Borrelia
burgdorferi in Mouse Tissues
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(801K)
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Section E Genomic Methods
- Unit 1E.1 Characterization of Bacteria in
Mixed Biofilm Communities Using Denaturing Gradient Gel Electrophoresis
(DGGE)
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(213K)
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Section F Proteomic Methods
- Unit 1F.2 Proteomic Approaches to Antibiotic
Drug Discovery
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(795K)
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Section G Bioremediation
- Unit 1G.1 Biological Sand Filters: Low-Cost
Bioremediation Technique for Production of Clean Drinking Water
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(538K)
Chapter 2 Microscopy
Chapter 3 Alpha Proteobacteria
- Introduction
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(25K)
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Section A Rickettsiales
- Unit 3A.1 Laboratory Maintenance of
Ehrlichia chaffeensis and
Ehrlichia canis and Recovery of Organisms
for Molecular Biology and Proteomics Studies
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(436K)
- Unit 3A.2 Laboratory Maintenance of
Anaplasma phagocytophilum
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(439K)
- Unit 3A.3 Isolation and Molecular Detection
of Ehrlichia from Vertebrate Animals
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(272K)
- Unit 3A.4 Transfection of
Wolbachia pipientis into Drosophila
Embryos
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(334K)
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Section B Brucella
- Unit 3B.1 Laboratory Maintenance of
Brucella abortus
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(153K)
Chapter 4 Beta Proteobacteria
Chapter 5 Enteric Gamma Proteobacteria
- Introduction
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(21K)
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Section A Escherichia coli
- Unit 5A.1 Interaction of Enterohemorrhagic
Escherichia coli (EHEC) with Mammalian Cells:
Cell Adhesion, Type III Secretion, and Actin Pedestal Formation
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(192K)
- Unit 5A.2 Rapid Allelic Exchange in
Enterohemorrhagic Escherichia coli (EHEC)
and Other E. coli Using
λ Red Recombination
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(153K)
Chapter 6 Nonenteric Gamma Proteobacteria
Chapter 7 Delta Proteobacteria
Chapter 8 Epsilon Proteobacteria
Chapter 9 Firmicutes (Low G+C Gram Positive)
Chapter 10 Actinobacteria (High G+C Gram Positive)
Chapter 11 Chlamydiae
Chapter 12 Spirochetes
Chapter 13 Other Eubacteria
Chapter 14 Animal DNA Viruses
Chapter 15 Animal RNA Viruses
- Introduction
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(27K)
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Section A Arenaviruses
- Unit 15A.1 Lymphocytic Choriomeningitis
Virus (LCMV): Propagation, Quantitation, and Storage
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(100K)
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Section B Alphaviruses
- Unit 15B.1 Sindbis Virus: Propagation,
Quantification, and Storage
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(378K)
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Section F Paramyxoviruses
- Unit 15F.2 Newcastle Disease Virus:
Propagation, Quantification, and Storage
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(394K)
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Section G Orthomyxoviruses
- Unit 15G.1 Influenza: Propagation,
Quantification, and Storage
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(298K)
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Section J Retroviruses
- Unit 15J.1 Human Immunodeficiency Viruses:
Propagation, Quantification, and Storage
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(493K)
Chapter 16 Plant RNA Viruses
Appendix 1 Useful Information and Data
- 1A Abbreviations Used in this Manual
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(52K)
- 1B Resources for International Biosafety
Guidelines and Regulations
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(34K)
Appendix 2 Commonly Used Reagents and Equipment
Appendix 3 Commonly Used Techniques
- 3A Assays for Total Protein
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- 3B Diagnosis and Treatment of Mycoplasma-Contaminated
Cell Cultures
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- 3C Gram Staining
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- 3D Direct PCR of Intact Bacteria (Colony PCR)
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(117K)
Appendix 4 Commonly Used Methods for Cell Culture
- 4A Common Bacterial Culture Techniques and Media
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(96K)
- 4B Isolation and Culture of Human Umbilical Vein
Endothelial Cells (HUVEC)
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(162K)
- 4C Isolation of Human Peripheral Blood
Mononuclear Cells (PBMCs)
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(317K)
